A rapid microfluidic platform with real-time fluorescence detection system for molecular diagnosis
نویسندگان
چکیده
منابع مشابه
A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
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متن کاملA rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
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در این پروژه هدف طراحی و ساخت fluorescence detection systemجهت آشکارسازی مولکول های dna می باشد. در روش pcr، طی چندین چرخه دمایی، مولکول های dna کپی می شوند و سپس با استفاده از روش fluorescence detection، مولکول های بر انگیخته شده dna آشکارسازی می شوند. پس از آشکارسازی می توان به کپی و یا عدم کپی dnaها پی برد.می توان برای جداسازی مولکول های dna بر اساس طولشان، از روش gel electrophoresis استفاده...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2019
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2018.1561211